polyclonal rabbit anti glun2d subunit Search Results


polyclonal rabbit anti glun2d subunit  (Alomone Labs)
94
Alomone Labs polyclonal rabbit anti glun2d subunit
(A) Grin2d f l/fl mice were injected with AAV5-CamKII-mCherry (Control) or AAV-CamKII-mCherry-Cre ( Grin2d cKO). NMDAR-LTP was abolished in Grin2d cKO compared with control mice (Control: 149.5 ± 6.0 %, p < 0.01, n = 5, paired t-test; cKO: 92.5 ± 5.3 %, p = 0.12201, n = 6, paired t-test; Control vs cKO: p < 0.001, unpaired t-test). (B) WT mice were bilaterally injected with <t>an</t> <t>anti-GluN2D</t> antibody or control Ab into the dentate gyrus. After one hour, animals were euthanized, and slices were prepared. Injection was confirmed by the presence of methylene blue. NMDAR-LTP was abolished in mice injected with the anti-GluN2D antibody (cKO: 110.4 ± 8.5 %, p = 0.2952, n = 6, paired t-test) compared with control mice (Control: 149.8 ± 8.1 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.01, unpaired t-test). (C) NMDAR-LTP was impaired in Grid1 KO mice (KO: 117.7 ± 5.3, p < 0.05%, n = 8, Wilcoxon signed-rank test) compared with controls (Control: 147.5 ± 6.7 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.05, Mann-Whitney U test). Data are presented as mean ± s.e.m.
Polyclonal Rabbit Anti Glun2d Subunit, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+rabbit+anti+glun2d+subunit/bio_rxiv__64898__2026__03__06__710109-151-27-31?v=Alomone+Labs
Average 94 stars, based on 1 article reviews
polyclonal rabbit anti glun2d subunit - by Bioz Stars, 2026-08
94/100 stars
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rabbit anti glun2d  (OriGene)
90
OriGene rabbit anti glun2d
(A) Grin2d f l/fl mice were injected with AAV5-CamKII-mCherry (Control) or AAV-CamKII-mCherry-Cre ( Grin2d cKO). NMDAR-LTP was abolished in Grin2d cKO compared with control mice (Control: 149.5 ± 6.0 %, p < 0.01, n = 5, paired t-test; cKO: 92.5 ± 5.3 %, p = 0.12201, n = 6, paired t-test; Control vs cKO: p < 0.001, unpaired t-test). (B) WT mice were bilaterally injected with <t>an</t> <t>anti-GluN2D</t> antibody or control Ab into the dentate gyrus. After one hour, animals were euthanized, and slices were prepared. Injection was confirmed by the presence of methylene blue. NMDAR-LTP was abolished in mice injected with the anti-GluN2D antibody (cKO: 110.4 ± 8.5 %, p = 0.2952, n = 6, paired t-test) compared with control mice (Control: 149.8 ± 8.1 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.01, unpaired t-test). (C) NMDAR-LTP was impaired in Grid1 KO mice (KO: 117.7 ± 5.3, p < 0.05%, n = 8, Wilcoxon signed-rank test) compared with controls (Control: 147.5 ± 6.7 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.05, Mann-Whitney U test). Data are presented as mean ± s.e.m.
Rabbit Anti Glun2d, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+rabbit+anti+glun2d+subunit/pmc07005343-406-57-65?v=OriGene
Average 90 stars, based on 1 article reviews
rabbit anti glun2d - by Bioz Stars, 2026-08
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rabbit anti nmdar antibodies  (Boster Bio)
86
Boster Bio rabbit anti nmdar antibodies
(A) Grin2d f l/fl mice were injected with AAV5-CamKII-mCherry (Control) or AAV-CamKII-mCherry-Cre ( Grin2d cKO). NMDAR-LTP was abolished in Grin2d cKO compared with control mice (Control: 149.5 ± 6.0 %, p < 0.01, n = 5, paired t-test; cKO: 92.5 ± 5.3 %, p = 0.12201, n = 6, paired t-test; Control vs cKO: p < 0.001, unpaired t-test). (B) WT mice were bilaterally injected with <t>an</t> <t>anti-GluN2D</t> antibody or control Ab into the dentate gyrus. After one hour, animals were euthanized, and slices were prepared. Injection was confirmed by the presence of methylene blue. NMDAR-LTP was abolished in mice injected with the anti-GluN2D antibody (cKO: 110.4 ± 8.5 %, p = 0.2952, n = 6, paired t-test) compared with control mice (Control: 149.8 ± 8.1 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.01, unpaired t-test). (C) NMDAR-LTP was impaired in Grid1 KO mice (KO: 117.7 ± 5.3, p < 0.05%, n = 8, Wilcoxon signed-rank test) compared with controls (Control: 147.5 ± 6.7 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.05, Mann-Whitney U test). Data are presented as mean ± s.e.m.
Rabbit Anti Nmdar Antibodies, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+rabbit+anti+glun2d+subunit/pm28944854-57-5-11?v=Boster+Bio
Average 86 stars, based on 1 article reviews
rabbit anti nmdar antibodies - by Bioz Stars, 2026-08
86/100 stars
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anti-nmdar2b (glun2b) (extracellular) antibody  (Alomone Labs)
95
Alomone Labs anti-nmdar2b (glun2b) (extracellular) antibody
(A) Grin2d f l/fl mice were injected with AAV5-CamKII-mCherry (Control) or AAV-CamKII-mCherry-Cre ( Grin2d cKO). NMDAR-LTP was abolished in Grin2d cKO compared with control mice (Control: 149.5 ± 6.0 %, p < 0.01, n = 5, paired t-test; cKO: 92.5 ± 5.3 %, p = 0.12201, n = 6, paired t-test; Control vs cKO: p < 0.001, unpaired t-test). (B) WT mice were bilaterally injected with <t>an</t> <t>anti-GluN2D</t> antibody or control Ab into the dentate gyrus. After one hour, animals were euthanized, and slices were prepared. Injection was confirmed by the presence of methylene blue. NMDAR-LTP was abolished in mice injected with the anti-GluN2D antibody (cKO: 110.4 ± 8.5 %, p = 0.2952, n = 6, paired t-test) compared with control mice (Control: 149.8 ± 8.1 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.01, unpaired t-test). (C) NMDAR-LTP was impaired in Grid1 KO mice (KO: 117.7 ± 5.3, p < 0.05%, n = 8, Wilcoxon signed-rank test) compared with controls (Control: 147.5 ± 6.7 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.05, Mann-Whitney U test). Data are presented as mean ± s.e.m.
Anti Nmdar2b (Glun2b) (Extracellular) Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+rabbit+anti+glun2d+subunit/custom%40agc-003%4010%2E1101%2F2024%2E06%2E06%2E597701?v=Alomone+Labs
Average 95 stars, based on 1 article reviews
anti-nmdar2b (glun2b) (extracellular) antibody - by Bioz Stars, 2026-08
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mouse anti-th  (ImmunoStar inc)
90
ImmunoStar inc mouse anti-th
(A) Grin2d f l/fl mice were injected with AAV5-CamKII-mCherry (Control) or AAV-CamKII-mCherry-Cre ( Grin2d cKO). NMDAR-LTP was abolished in Grin2d cKO compared with control mice (Control: 149.5 ± 6.0 %, p < 0.01, n = 5, paired t-test; cKO: 92.5 ± 5.3 %, p = 0.12201, n = 6, paired t-test; Control vs cKO: p < 0.001, unpaired t-test). (B) WT mice were bilaterally injected with <t>an</t> <t>anti-GluN2D</t> antibody or control Ab into the dentate gyrus. After one hour, animals were euthanized, and slices were prepared. Injection was confirmed by the presence of methylene blue. NMDAR-LTP was abolished in mice injected with the anti-GluN2D antibody (cKO: 110.4 ± 8.5 %, p = 0.2952, n = 6, paired t-test) compared with control mice (Control: 149.8 ± 8.1 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.01, unpaired t-test). (C) NMDAR-LTP was impaired in Grid1 KO mice (KO: 117.7 ± 5.3, p < 0.05%, n = 8, Wilcoxon signed-rank test) compared with controls (Control: 147.5 ± 6.7 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.05, Mann-Whitney U test). Data are presented as mean ± s.e.m.
Mouse Anti Th, supplied by ImmunoStar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+rabbit+anti+glun2d+subunit/bio_rxiv__2024__06__06__597701-82-13-16?v=ImmunoStar+inc
Average 90 stars, based on 1 article reviews
mouse anti-th - by Bioz Stars, 2026-08
90/100 stars
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anti-β-actin  (Delta Biolabs)
90
Delta Biolabs anti-β-actin
(A) Grin2d f l/fl mice were injected with AAV5-CamKII-mCherry (Control) or AAV-CamKII-mCherry-Cre ( Grin2d cKO). NMDAR-LTP was abolished in Grin2d cKO compared with control mice (Control: 149.5 ± 6.0 %, p < 0.01, n = 5, paired t-test; cKO: 92.5 ± 5.3 %, p = 0.12201, n = 6, paired t-test; Control vs cKO: p < 0.001, unpaired t-test). (B) WT mice were bilaterally injected with <t>an</t> <t>anti-GluN2D</t> antibody or control Ab into the dentate gyrus. After one hour, animals were euthanized, and slices were prepared. Injection was confirmed by the presence of methylene blue. NMDAR-LTP was abolished in mice injected with the anti-GluN2D antibody (cKO: 110.4 ± 8.5 %, p = 0.2952, n = 6, paired t-test) compared with control mice (Control: 149.8 ± 8.1 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.01, unpaired t-test). (C) NMDAR-LTP was impaired in Grid1 KO mice (KO: 117.7 ± 5.3, p < 0.05%, n = 8, Wilcoxon signed-rank test) compared with controls (Control: 147.5 ± 6.7 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.05, Mann-Whitney U test). Data are presented as mean ± s.e.m.
Anti β Actin, supplied by Delta Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+rabbit+anti+glun2d+subunit/pm29454156-62-50-52?v=Delta+Biolabs
Average 90 stars, based on 1 article reviews
anti-β-actin - by Bioz Stars, 2026-08
90/100 stars
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map2/map-2a.b.c polyclonal antibody  (Bioss)
94
Bioss map2/map-2a.b.c polyclonal antibody
(A) Grin2d f l/fl mice were injected with AAV5-CamKII-mCherry (Control) or AAV-CamKII-mCherry-Cre ( Grin2d cKO). NMDAR-LTP was abolished in Grin2d cKO compared with control mice (Control: 149.5 ± 6.0 %, p < 0.01, n = 5, paired t-test; cKO: 92.5 ± 5.3 %, p = 0.12201, n = 6, paired t-test; Control vs cKO: p < 0.001, unpaired t-test). (B) WT mice were bilaterally injected with <t>an</t> <t>anti-GluN2D</t> antibody or control Ab into the dentate gyrus. After one hour, animals were euthanized, and slices were prepared. Injection was confirmed by the presence of methylene blue. NMDAR-LTP was abolished in mice injected with the anti-GluN2D antibody (cKO: 110.4 ± 8.5 %, p = 0.2952, n = 6, paired t-test) compared with control mice (Control: 149.8 ± 8.1 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.01, unpaired t-test). (C) NMDAR-LTP was impaired in Grid1 KO mice (KO: 117.7 ± 5.3, p < 0.05%, n = 8, Wilcoxon signed-rank test) compared with controls (Control: 147.5 ± 6.7 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.05, Mann-Whitney U test). Data are presented as mean ± s.e.m.
Map2/Map 2a.B.C Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+rabbit+anti+glun2d+subunit/custom%40bs-1369r%4010%2E1172%2Fjci88170?v=Bioss
Average 94 stars, based on 1 article reviews
map2/map-2a.b.c polyclonal antibody - by Bioz Stars, 2026-08
94/100 stars
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anti-glun1  (Becton Dickinson)
90
Becton Dickinson anti-glun1
(A) Grin2d f l/fl mice were injected with AAV5-CamKII-mCherry (Control) or AAV-CamKII-mCherry-Cre ( Grin2d cKO). NMDAR-LTP was abolished in Grin2d cKO compared with control mice (Control: 149.5 ± 6.0 %, p < 0.01, n = 5, paired t-test; cKO: 92.5 ± 5.3 %, p = 0.12201, n = 6, paired t-test; Control vs cKO: p < 0.001, unpaired t-test). (B) WT mice were bilaterally injected with <t>an</t> <t>anti-GluN2D</t> antibody or control Ab into the dentate gyrus. After one hour, animals were euthanized, and slices were prepared. Injection was confirmed by the presence of methylene blue. NMDAR-LTP was abolished in mice injected with the anti-GluN2D antibody (cKO: 110.4 ± 8.5 %, p = 0.2952, n = 6, paired t-test) compared with control mice (Control: 149.8 ± 8.1 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.01, unpaired t-test). (C) NMDAR-LTP was impaired in Grid1 KO mice (KO: 117.7 ± 5.3, p < 0.05%, n = 8, Wilcoxon signed-rank test) compared with controls (Control: 147.5 ± 6.7 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.05, Mann-Whitney U test). Data are presented as mean ± s.e.m.
Anti Glun1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+rabbit+anti+glun2d+subunit/pm29454156-62-21-23?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
anti-glun1 - by Bioz Stars, 2026-08
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rabbit anti-glun2c  (Abnova)
90
Abnova rabbit anti-glun2c
(A) Grin2d f l/fl mice were injected with AAV5-CamKII-mCherry (Control) or AAV-CamKII-mCherry-Cre ( Grin2d cKO). NMDAR-LTP was abolished in Grin2d cKO compared with control mice (Control: 149.5 ± 6.0 %, p < 0.01, n = 5, paired t-test; cKO: 92.5 ± 5.3 %, p = 0.12201, n = 6, paired t-test; Control vs cKO: p < 0.001, unpaired t-test). (B) WT mice were bilaterally injected with <t>an</t> <t>anti-GluN2D</t> antibody or control Ab into the dentate gyrus. After one hour, animals were euthanized, and slices were prepared. Injection was confirmed by the presence of methylene blue. NMDAR-LTP was abolished in mice injected with the anti-GluN2D antibody (cKO: 110.4 ± 8.5 %, p = 0.2952, n = 6, paired t-test) compared with control mice (Control: 149.8 ± 8.1 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.01, unpaired t-test). (C) NMDAR-LTP was impaired in Grid1 KO mice (KO: 117.7 ± 5.3, p < 0.05%, n = 8, Wilcoxon signed-rank test) compared with controls (Control: 147.5 ± 6.7 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.05, Mann-Whitney U test). Data are presented as mean ± s.e.m.
Rabbit Anti Glun2c, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+rabbit+anti+glun2d+subunit/pmc07005343-406-46-53?v=Abnova
Average 90 stars, based on 1 article reviews
rabbit anti-glun2c - by Bioz Stars, 2026-08
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anti-α-tubulin  (Biozol Diagnostica Vertrieb GmbH)
90
Biozol Diagnostica Vertrieb GmbH anti-α-tubulin
(A) Grin2d f l/fl mice were injected with AAV5-CamKII-mCherry (Control) or AAV-CamKII-mCherry-Cre ( Grin2d cKO). NMDAR-LTP was abolished in Grin2d cKO compared with control mice (Control: 149.5 ± 6.0 %, p < 0.01, n = 5, paired t-test; cKO: 92.5 ± 5.3 %, p = 0.12201, n = 6, paired t-test; Control vs cKO: p < 0.001, unpaired t-test). (B) WT mice were bilaterally injected with <t>an</t> <t>anti-GluN2D</t> antibody or control Ab into the dentate gyrus. After one hour, animals were euthanized, and slices were prepared. Injection was confirmed by the presence of methylene blue. NMDAR-LTP was abolished in mice injected with the anti-GluN2D antibody (cKO: 110.4 ± 8.5 %, p = 0.2952, n = 6, paired t-test) compared with control mice (Control: 149.8 ± 8.1 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.01, unpaired t-test). (C) NMDAR-LTP was impaired in Grid1 KO mice (KO: 117.7 ± 5.3, p < 0.05%, n = 8, Wilcoxon signed-rank test) compared with controls (Control: 147.5 ± 6.7 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.05, Mann-Whitney U test). Data are presented as mean ± s.e.m.
Anti α Tubulin, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+rabbit+anti+glun2d+subunit/pm29454156-62-56-58?v=Biozol+Diagnostica+Vertrieb+GmbH
Average 90 stars, based on 1 article reviews
anti-α-tubulin - by Bioz Stars, 2026-08
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rabbit polyclonal anti-glun3a  (Merck KGaA)
90
Merck KGaA rabbit polyclonal anti-glun3a
CNS endothelial NMDARs display the regulatory GluN2B and <t>GluN3A</t> subunits. Confocal photomicrographs represent coimmunostaining for the GluN1 subunit (anti-N-terminal domain; red) with the following regulatory NMDAR subunit (green): (A) Glun2B, GluN3A (corresponding 3D reconstruction in B), (C) GluN2A, GluN2C, GluN2D, and GluN3B in ECs of the mouse spinal cord. Yellow represents colocalizations. Scale bar, 10 μm.
Rabbit Polyclonal Anti Glun3a, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
rabbit polyclonal anti-glun3a - by Bioz Stars, 2026-08
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rabbit anti glun1  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc rabbit anti glun1
CNS endothelial NMDARs display the regulatory GluN2B and <t>GluN3A</t> subunits. Confocal photomicrographs represent coimmunostaining for the GluN1 subunit (anti-N-terminal domain; red) with the following regulatory NMDAR subunit (green): (A) Glun2B, GluN3A (corresponding 3D reconstruction in B), (C) GluN2A, GluN2C, GluN2D, and GluN3B in ECs of the mouse spinal cord. Yellow represents colocalizations. Scale bar, 10 μm.
Rabbit Anti Glun1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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Image Search Results


(A) Grin2d f l/fl mice were injected with AAV5-CamKII-mCherry (Control) or AAV-CamKII-mCherry-Cre ( Grin2d cKO). NMDAR-LTP was abolished in Grin2d cKO compared with control mice (Control: 149.5 ± 6.0 %, p < 0.01, n = 5, paired t-test; cKO: 92.5 ± 5.3 %, p = 0.12201, n = 6, paired t-test; Control vs cKO: p < 0.001, unpaired t-test). (B) WT mice were bilaterally injected with an anti-GluN2D antibody or control Ab into the dentate gyrus. After one hour, animals were euthanized, and slices were prepared. Injection was confirmed by the presence of methylene blue. NMDAR-LTP was abolished in mice injected with the anti-GluN2D antibody (cKO: 110.4 ± 8.5 %, p = 0.2952, n = 6, paired t-test) compared with control mice (Control: 149.8 ± 8.1 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.01, unpaired t-test). (C) NMDAR-LTP was impaired in Grid1 KO mice (KO: 117.7 ± 5.3, p < 0.05%, n = 8, Wilcoxon signed-rank test) compared with controls (Control: 147.5 ± 6.7 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.05, Mann-Whitney U test). Data are presented as mean ± s.e.m.

Journal: bioRxiv

Article Title: GluN2D-containing NMDA receptors regulate dentate gyrus function by facilitating granule cell activity and mediating synaptic plasticity

doi: 10.64898/2026.03.06.710109

Figure Lengend Snippet: (A) Grin2d f l/fl mice were injected with AAV5-CamKII-mCherry (Control) or AAV-CamKII-mCherry-Cre ( Grin2d cKO). NMDAR-LTP was abolished in Grin2d cKO compared with control mice (Control: 149.5 ± 6.0 %, p < 0.01, n = 5, paired t-test; cKO: 92.5 ± 5.3 %, p = 0.12201, n = 6, paired t-test; Control vs cKO: p < 0.001, unpaired t-test). (B) WT mice were bilaterally injected with an anti-GluN2D antibody or control Ab into the dentate gyrus. After one hour, animals were euthanized, and slices were prepared. Injection was confirmed by the presence of methylene blue. NMDAR-LTP was abolished in mice injected with the anti-GluN2D antibody (cKO: 110.4 ± 8.5 %, p = 0.2952, n = 6, paired t-test) compared with control mice (Control: 149.8 ± 8.1 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.01, unpaired t-test). (C) NMDAR-LTP was impaired in Grid1 KO mice (KO: 117.7 ± 5.3, p < 0.05%, n = 8, Wilcoxon signed-rank test) compared with controls (Control: 147.5 ± 6.7 %, p < 0.001, n = 7, paired t-test; Control vs cKO: p < 0.05, Mann-Whitney U test). Data are presented as mean ± s.e.m.

Article Snippet: For GluN2D cross-linking experiments in C57BL/6J, the control group received 1 μL of anti-rabbit Alexa 568 (control IgG, 1/5), while the GluN2D-cross-link group received 1 μg of polyclonal rabbit anti-GluN2D subunit (Alomone Labs, cat #AGC-020), both diluted in PBS with 1% methylene blue (1 μL final volume).

Techniques: Injection, Control, MANN-WHITNEY

CNS endothelial NMDARs display the regulatory GluN2B and GluN3A subunits. Confocal photomicrographs represent coimmunostaining for the GluN1 subunit (anti-N-terminal domain; red) with the following regulatory NMDAR subunit (green): (A) Glun2B, GluN3A (corresponding 3D reconstruction in B), (C) GluN2A, GluN2C, GluN2D, and GluN3B in ECs of the mouse spinal cord. Yellow represents colocalizations. Scale bar, 10 μm.

Journal: The Journal of Neuroscience

Article Title: Nonionotropic Action of Endothelial NMDA Receptors on Blood–Brain Barrier Permeability via Rho/ROCK-Mediated Phosphorylation of Myosin

doi: 10.1523/JNEUROSCI.0969-19.2019

Figure Lengend Snippet: CNS endothelial NMDARs display the regulatory GluN2B and GluN3A subunits. Confocal photomicrographs represent coimmunostaining for the GluN1 subunit (anti-N-terminal domain; red) with the following regulatory NMDAR subunit (green): (A) Glun2B, GluN3A (corresponding 3D reconstruction in B), (C) GluN2A, GluN2C, GluN2D, and GluN3B in ECs of the mouse spinal cord. Yellow represents colocalizations. Scale bar, 10 μm.

Article Snippet: To analyze NMDAR subunits and tight junction proteins, the spinal cord tissue sample sections were incubated with the following primary antibodies: mouse monoclonal anti-GluN1 (Glunomab, 1:500) ( Lesept et al., 2016 ; Macrez et al., 2016 ), goat polyclonal anti-GluN2A (#1468, Santa Cruz Biotechnology, 1:200), rabbit polyclonal anti-GluN2B (#9057, Santa Cruz Biotechnology, 1:200), goat polyclonal anti-GluN2C (#1470, Santa Cruz Biotechnology, 1:200), goat polyclonal anti-GluN2D (#31551, Santa Cruz Biotechnology, 1:200), rabbit polyclonal anti-GluN3A (#07-356, Merck Millipore, 1:200), rabbit polyclonal anti-GluN3B (#50474, Santa Cruz Biotechnology, 1:200), rabbit monoclonal anti-claudin-5 (#131259, Abcam, 1:1000), rabbit monoclonal anti-occludin (#167161, Abcam, 1:1000), rabbit polyclonal anti-ZO-1 (zonula occludens -1 #59720, Abcam, 1:1000), rabbit polyclonal anti-VE-Cadherin (vascular endothelial cadherin #33168, Abcam, 1:1000), and goat anti-collagen IV antibody (#1340, Southern Biotechnology, 1:1000).

Techniques:

Inflammation-like conditions modulate the expression of NMDAR in hCMEC/D3 cells. A, Photomicrographs of hCMEC/D3 cells represent immunostaining for the NMDAR subunits GluN1, GluN2B, and GluN3A in control conditions or after TNF treatment (1 ng/ml). Scale bar, 20 μm. B, Corresponding quantification. Histograms represent mean ± SEM immunofluorescence. ****p < 0.0001. C, Immunoblotting against GluN1, GluN2B, and GluN3A of proteins extracted from hCMEC/D3 cells in control conditions or after TNF treatment and corresponding quantification. Histograms represent mean ± SEM (n = 3). *p < 0.05. **p < 0.01.

Journal: The Journal of Neuroscience

Article Title: Nonionotropic Action of Endothelial NMDA Receptors on Blood–Brain Barrier Permeability via Rho/ROCK-Mediated Phosphorylation of Myosin

doi: 10.1523/JNEUROSCI.0969-19.2019

Figure Lengend Snippet: Inflammation-like conditions modulate the expression of NMDAR in hCMEC/D3 cells. A, Photomicrographs of hCMEC/D3 cells represent immunostaining for the NMDAR subunits GluN1, GluN2B, and GluN3A in control conditions or after TNF treatment (1 ng/ml). Scale bar, 20 μm. B, Corresponding quantification. Histograms represent mean ± SEM immunofluorescence. ****p < 0.0001. C, Immunoblotting against GluN1, GluN2B, and GluN3A of proteins extracted from hCMEC/D3 cells in control conditions or after TNF treatment and corresponding quantification. Histograms represent mean ± SEM (n = 3). *p < 0.05. **p < 0.01.

Article Snippet: To analyze NMDAR subunits and tight junction proteins, the spinal cord tissue sample sections were incubated with the following primary antibodies: mouse monoclonal anti-GluN1 (Glunomab, 1:500) ( Lesept et al., 2016 ; Macrez et al., 2016 ), goat polyclonal anti-GluN2A (#1468, Santa Cruz Biotechnology, 1:200), rabbit polyclonal anti-GluN2B (#9057, Santa Cruz Biotechnology, 1:200), goat polyclonal anti-GluN2C (#1470, Santa Cruz Biotechnology, 1:200), goat polyclonal anti-GluN2D (#31551, Santa Cruz Biotechnology, 1:200), rabbit polyclonal anti-GluN3A (#07-356, Merck Millipore, 1:200), rabbit polyclonal anti-GluN3B (#50474, Santa Cruz Biotechnology, 1:200), rabbit monoclonal anti-claudin-5 (#131259, Abcam, 1:1000), rabbit monoclonal anti-occludin (#167161, Abcam, 1:1000), rabbit polyclonal anti-ZO-1 (zonula occludens -1 #59720, Abcam, 1:1000), rabbit polyclonal anti-VE-Cadherin (vascular endothelial cadherin #33168, Abcam, 1:1000), and goat anti-collagen IV antibody (#1340, Southern Biotechnology, 1:1000).

Techniques: Expressing, Immunostaining, Immunofluorescence, Western Blot